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Image Search Results
Journal: Molecular cell
Article Title: HOTTIP -dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia
doi: 10.1016/j.molcel.2022.01.014
Figure Lengend Snippet: Key resources table
Article Snippet:
Techniques: Control, Recombinant, CRISPR, Electroporation, Protease Inhibitor, Staining, Transfection, Reverse Transcription, Purification, Plasmid Preparation, DNA Library Preparation, Multiplex Assay, Hybridization, Library Quantification, Sensitive Assay, SYBR Green Assay, Transgenic Assay, Software
Journal: Journal of visualized experiments : JoVE
Article Title: Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
doi: 10.3791/62182
Figure Lengend Snippet: Outlines PCR primer sequences used for the detection of select genes involved in neonatal keratinocyte plasticity.
Article Snippet: Use GAPDH as an internal control ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name of Material/ Equipment Company Catalog Number Comments/Description MCDB 153-LB basal media Sigma-Aldrich M7403 MCDB 153-LB basal media w/ HEPES buffer KSFM-scm ThermoFisher Scientific 17005042 Supplemented with 1% Penicillin/Streptomycin, 20 ng/ml EGF, 10 ng/ml basic fibroblast growth factor, 0.4% bovine serum albumin (BSA), and 4 μg/ml insulin Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) Cell Signaling Technology 8910 Human Epidermal Growth Factor (hEGF) Cell Signaling Technology 8916 Human Insulin Millipore Sigma 9011-M Thermo Scientific™ Sterile Single Use Vacuum Filter Units Thermo Scientific 09-740-63D FITC-conjugated anti-integrinα6 Abcam ab30496 PE-conjugated anti-EGFR ( San Jose, CA; catalog number ) BD Pharmingen 555997 Dispase Sigma-Aldrich D4818 NEST Scientific 1-Well Cell Culture Chamber Slide, BLACK Walls on Glass Slide, 6/PK, 12/CS Stellar Scientific NST230111 P63 Thermo Scientific 703809 1:200 dilution Cytokeratin 14 Santa Cruz Biotechnology sc-53253 1:200 dilution All
Techniques: Cell Culture, SYBR Green Assay, Plasmid Preparation